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A Schematic representation of the SARS-CoV-2 RT-RPA-LUNAS assay, showing the viral genome and zooming in on the assay target region (ORF1a_1) within ORF1a (see Supplementary Table 1 for primer and gRNA sequences). B Intensiometric one-pot RT-RPA-LUNAS response over time for a range of IVT ORF1a RNA inputs (see legend at (C) ). C Ratiometric one-pot RT-RPA-LUNAS response over time for a range of IVT ORF1a RNA inputs. D Luminescence spectra of a single ratiometric one-pot RT-RPA-LUNAS reaction from panel ( C ) (200 cp input), showing one line per timepoint over the first 45 min, with trends indicated by arrows (see also Supplementary Fig. 8). E Ratiometric one-pot RT-RPA-LUNAS response over time for genomic RNA (2 × 10 4 cp) isolated from SARS-CoV-2 (target) and from closely <t>related</t> <t>HCoV-229E</t> and HCoV-OC43 non-target viruses, showing the specificity for the target. F One-pot RT-RPA-LUNAS compatibility tests for eSwab, VTM and saliva sample matrices, showing ratiometric RT-RPA-LUNAS response over time for the same range of IVT ORF1a inputs as in ( D ). Mixtures of the sample media and inactivation buffer (1:1) were spiked with IVT target RNA and heated at 95°C for 5 min (eSwab, saliva) or 70°C for 10 min (VTM) (see methods) before addition to RT-RPA-LUNAS reactions. In ( B, C, E, F ) individual replicate traces ( n = 3) are shown. NTC: No template control.
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Image Search Results


Coronavirus antigens on microarray.

Journal: bioRxiv

Article Title: Analysis of Serologic Cross-Reactivity Between Common Human Coronaviruses and SARS-CoV-2 Using Coronavirus Antigen Microarray

doi: 10.1101/2020.03.24.006544

Figure Lengend Snippet: Coronavirus antigens on microarray.

Article Snippet: Coronavirus , 229E , 229E , S1+S2 , A0A1L7B942 , Insect Cells , Sino Biological , N-(AA16-1115)-His-C , 40605-V08B.

Techniques: Microarray, Expressing, Construct

Coronavirus antigens on microarray.

Journal: bioRxiv

Article Title: Analysis of Serologic Cross-Reactivity Between Common Human Coronaviruses and SARS-CoV-2 Using Coronavirus Antigen Microarray

doi: 10.1101/2020.03.24.006544

Figure Lengend Snippet: Coronavirus antigens on microarray.

Article Snippet: Coronavirus , HKU1 , HKU1 , S1 , Q0ZME7 , HEK293 , Sino Biological , N-(AA13-756)-His-C , 40602-V08H.

Techniques: Microarray, Expressing, Construct

A Schematic representation of the SARS-CoV-2 RT-RPA-LUNAS assay, showing the viral genome and zooming in on the assay target region (ORF1a_1) within ORF1a (see Supplementary Table 1 for primer and gRNA sequences). B Intensiometric one-pot RT-RPA-LUNAS response over time for a range of IVT ORF1a RNA inputs (see legend at (C) ). C Ratiometric one-pot RT-RPA-LUNAS response over time for a range of IVT ORF1a RNA inputs. D Luminescence spectra of a single ratiometric one-pot RT-RPA-LUNAS reaction from panel ( C ) (200 cp input), showing one line per timepoint over the first 45 min, with trends indicated by arrows (see also Supplementary Fig. 8). E Ratiometric one-pot RT-RPA-LUNAS response over time for genomic RNA (2 × 10 4 cp) isolated from SARS-CoV-2 (target) and from closely related HCoV-229E and HCoV-OC43 non-target viruses, showing the specificity for the target. F One-pot RT-RPA-LUNAS compatibility tests for eSwab, VTM and saliva sample matrices, showing ratiometric RT-RPA-LUNAS response over time for the same range of IVT ORF1a inputs as in ( D ). Mixtures of the sample media and inactivation buffer (1:1) were spiked with IVT target RNA and heated at 95°C for 5 min (eSwab, saliva) or 70°C for 10 min (VTM) (see methods) before addition to RT-RPA-LUNAS reactions. In ( B, C, E, F ) individual replicate traces ( n = 3) are shown. NTC: No template control.

Journal: bioRxiv

Article Title: Fast bioluminescent nucleic acid detection using one-pot isothermal amplification and dCas9-based split luciferase complementation

doi: 10.1101/2022.09.12.507659

Figure Lengend Snippet: A Schematic representation of the SARS-CoV-2 RT-RPA-LUNAS assay, showing the viral genome and zooming in on the assay target region (ORF1a_1) within ORF1a (see Supplementary Table 1 for primer and gRNA sequences). B Intensiometric one-pot RT-RPA-LUNAS response over time for a range of IVT ORF1a RNA inputs (see legend at (C) ). C Ratiometric one-pot RT-RPA-LUNAS response over time for a range of IVT ORF1a RNA inputs. D Luminescence spectra of a single ratiometric one-pot RT-RPA-LUNAS reaction from panel ( C ) (200 cp input), showing one line per timepoint over the first 45 min, with trends indicated by arrows (see also Supplementary Fig. 8). E Ratiometric one-pot RT-RPA-LUNAS response over time for genomic RNA (2 × 10 4 cp) isolated from SARS-CoV-2 (target) and from closely related HCoV-229E and HCoV-OC43 non-target viruses, showing the specificity for the target. F One-pot RT-RPA-LUNAS compatibility tests for eSwab, VTM and saliva sample matrices, showing ratiometric RT-RPA-LUNAS response over time for the same range of IVT ORF1a inputs as in ( D ). Mixtures of the sample media and inactivation buffer (1:1) were spiked with IVT target RNA and heated at 95°C for 5 min (eSwab, saliva) or 70°C for 10 min (VTM) (see methods) before addition to RT-RPA-LUNAS reactions. In ( B, C, E, F ) individual replicate traces ( n = 3) are shown. NTC: No template control.

Article Snippet: In addition, SARS-CoV-2 (Isolate USA-WA1/2020, BEI catalog No. NR-52347), HCoV-OC43 (BEI catalog No. NR-52727) and HCoV-229E (BEI catalog No. NR-52728) genomic RNA extracted from heat-inactivated virus was obtained from BEI Resources at known concentrations as determined by ddPCR.

Techniques: Isolation